find.genomic.events: Finds the genomic events associated with each of the array...

In aCGH: Classes and functions for Array Comparative Genomic Hybridization data

Description

Finds the genomic events associated with each of the array CGH samples. Events include whole chromosomal gains and losses, aberrations, transitions, amplifications and their respective counts and sizes. The hmm states has to be computed before using this function.

Usage

find.genomic.events(aCGH.obj, maxChrom = 23, factor = 5, maxClones = 1,
                    maxLen = 1000, absValSingle = 1, absValRegion = 1,
                    diffVal1 = 1, diffVal2 = .5, maxSize = 10000,
                    pChrom.min = .9, medChrom.min = .1)

Arguments

aCGH.obj	Object of class aCGH.
maxChrom	Highest chromosomal number to find events.
factor	Determines outliers. See findOutliers.func.
maxClones	Determines aberrations. See findAber.func.
maxLen	Determines aberrations. See findAber.func.
absValSingle	Determines amplifications. See findAmplif.func.
absValRegion	Determines amplifications. See findAmplif.func.
diffVal1	Determines amplifications. See findAmplif.func.
diffVal2	Determines amplifications. See findAmplif.func.
maxSize	Determines amplifications. See findAmplif.func.
pChrom.min	Determines whole chromosomal gains and losses. Chromosome should contain no transitions, have its absolute median equal or greater than medChrom.min and at least medChrom.min has to be greater or less than 0.
medChrom.min	Determines whole chromosomal gains and losses. Chromosome should contain no transitions, have its absolute median equal or greater than medChrom.min and at least medChrom.min has to be greater or less than 0.

Details

The default parameters generally work. Threshold for merging may be changed depending on the expected normal cell contamination and/or expected magnitude of the changes. AIC model generally works, however, may need to be readjusted depending on how liberal or conservative one wants to be in finding genomic events. We recommend BIC criterion with delta = 1 for noisier data.

Value

num.transitions	matrix of dimensions maxChrom by number of samples. It contains number of transitions that were recorded on a given chromosome for a given sample.
num.amplifications	matrix of dimensions maxChrom by number of samples It contains number of amplifications that were recorded on a given chromosome for a given sample.
num.aberrations	matrix of dimensions maxChrom by number of samples. It contains number of focal aberrations that were recorded on a given chromosome for a given sample.
num.outliers	matrix of dimensions maxChrom by number of samples. It contains number of outliers that were recorded on a given chromosome for a given sample.
num.transitions.binary	binary matrix of dimensions maxChrom by number of samples. Non-zero entry indicates whether 1 or more transitions were recorded on a given chromosome for a given sample.
num.amplifications.binary	binary matrix of dimensions maxChrom by number of samples. Non-zero entry indicates whether 1 or more amplifications were recorded on a given chromosome for a given sample.
num.aberrations.binary	binary matrix of dimensions maxChrom by number of samples. Non-zero entry indicates whether 1 or more focal aberrations were recorded on a given chromosome for a given sample.
num.outliers.binary	binary matrix of dimensions maxChrom by number of samples. Non-zero entry indicates whether 1 or more outliers were recorded on a given chromosome for a given sample.
whole.chrom.gain.loss	matrix of dimensions maxChrom by number of samples. Positive entry indicates that a given chromosome was gained in a given sample, negative entry indicates that a given chromosome was lost in a given sample, 0 entry is normal chromosome and NA marks chromosomes with one or more transition.
size.amplicons	matrix of dimensions maxChrom by number of samples. Reports size of a given chromosome that is amplified (kb units) in a given sample.
num.amplicons	matrix of dimensions maxChrom by number of samples. Reports number of disjoint amplicons on a given chromosome for a given sample.
outliers	list containing 3 matrices of dimensions number of clones by number of samples. See findOutliers.func.
aberrations	list containing a matrix of dimensions number of clones by number of samples. See findAber.func.
transitions	list containing 2 matrices of dimensions number of clones by number of samples. See findTrans.func.
amplifications	list containing a matrix of dimensions number of clones by number of samples. See findAmplif.func.

See Also

aCGH find.hmm.states mergeFunc findAber.func findTrans.func findAmplif.func findOutliers.func

aCGH documentation built on Nov. 8, 2020, 6:58 p.m.