Description Usage Arguments Value
View source: R/internal_fluo_est.R
A helper to re-estimate the spot location or find the capture site location (BF image modelling).
1 2 | getCsFAIL(centerR, centerG, origImg, chaImgs, minDiff, despeckle,
ImgLimits, BFarea, chip.type, separator, image.type)
|
centerR |
Data matrix. The location statistics of one channel. |
centerG |
Data matrix. The location statistics of the other channel. |
origImg |
Data matrix. The original BF image to be read and processed. |
chaImgs |
List. A list of the channel images (data matrices) of a sample. |
minDiff |
Float. the mu_hat of the H0: image-to-noise ratio = log(foreground_signal) - log(background_signal) = mu_hat. Rejection of H0 implies that the identified spot is brighter than background. |
despeckle |
Logical. If TRUE, the BF image is descpeckled. |
ImgLimits |
Integer. It instructs the algorithm to find spots in a certain central image area. For example, for a 512 x 512 image with ImgLimits = 50, it will search for spots in the central area [ImgLimits:(512-ImgLimits),ImgLimits:(512-ImgLimits)] of the image matrix. |
BFarea |
Integer. Defines a rectangular pseudo-spot size whose fluorescence will be estimated. |
chip.type |
Character string. It specifies the type of Fluidigm chip to be analyzed. |
separator |
Character string. Removes the Bright Field ("BF") and channel indicators from the image file names. |
image.type |
Character string. A triplet of IDs to characterize the type of images under study. They refer to the ImageType part of the original image or txt file names. |
Location statistics under BF image modelling
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