BatchFluo: BatchFluo

In CONFESS: Cell OrderiNg by FluorEScence Signal

Description

It performs the run effect correction of the cell fluorescence signals by flexmix or 2-way ANOVA.

Usage

BatchFluo(data, maxMix, reference, prior.pi, flex.reps, flexmethod, seed)

Arguments

data	List. A list of transformed and adjusted fluorescence signals.
maxMix	Integer. The maximum number of components to fit into the mixture of regressions model. If maxMix=1 or if the the optimal number of the estimated components is 1, the model reduces to the classical 2-way ANOVA.
reference	Numeric vector. Specifies the runs to be used as baseline (iteratively).
prior.pi	Float. The prior probability to accept a component.
flex.reps	Integer. The iterations of the Expectation-Maximization algorithm to estimate the flexmix model.
flexmethod	Character string. A method to estimate the optimal number of flexmix components. One of "BIC", "AIC", "ICL".
seed	Integer. An optional seed number for the Random Number Generator.

Value

A list of fluorescence signals, mixture components and flexmix model estimates

CONFESS documentation built on Nov. 8, 2020, 6:05 p.m.