Description Usage Arguments Value
It processes the case of 0 spots in both channels. It performs BF image modelling.
1 2 | caseof0s(centerR, centerG, origImg, chaImgs, minDiff, despeckle, ImgLimits,
BFarea, chip.type, separator, image.type)
|
centerR |
Data matrix. The location statistics of one channel. |
centerG |
Data matrix. The location statistics of the other channel. |
origImg |
Data matrix. The original BF image to be read and processed. |
chaImgs |
List. The channel image data (data matrices) of a sample. |
minDiff |
Float. the mu_hat of the H0: image-to-noise ratio = log(foreground_signal) - log(background_signal) = mu_hat. Rejection of H0 implies that the identified spot is brighter than background. Default is 0.5. |
despeckle |
Logical. If TRUE the BF image is despeckled. |
ImgLimits |
Integer. It instructs the algorithm to find spots in the specified central image area. For example, for a 512 x 512 image with cutSides = 50, it will search for spots in the central area [cutSides:(512-ImgLimits),ImgLimits:(512-ImgLimits)] of the image matrix. |
BFarea |
Integer. Defines a rectangular pseudo-spot size whose fluorescence will be estimated. This is mainly used in BF image modeling where a fluorescence spot could not be originally detected. The value of this parameter is also used as a cut-off to find matched spots across channel of the same sample image. |
chip.type |
Character string. It specifies the type of Fluidigm chip to be analyzed. |
separator |
Character string. Removes the Bright Field ("BF") and channel indicators (IDs) from the image file names. |
image.type |
Character string. A triplet of IDs to characterize the type of images under study. |
A list of location estimates
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