subDiffEx: Passes the output of generateSimulatedData() to differential...

In mmkhan19/singleCellTK: Interactive Analysis of Single Cell RNA-Seq Data

Passes the output of generateSimulatedData() to differential expression tests, picking either t-tests or ANOVA for data with only two conditions or multiple conditions, respectively.

Description

Passes the output of generateSimulatedData() to differential expression tests, picking either t-tests or ANOVA for data with only two conditions or multiple conditions, respectively.

Usage

subDiffEx(tempData)

subDiffExttest(countMatrix, class.labels, test.type = "t.equalvar")

subDiffExANOVA(countMatrix, condition)

Arguments

tempData	Matrix. The output of generateSimulatedData(), where the first row contains condition labels.
countMatrix	Matrix. A simulated counts matrix, sans labels.
class.labels	Factor. The condition labels for the simulated cells. Will be coerced into 1's and 0's.
test.type	Type of test to perform. The default is t.equalvar.
condition	Factor. The condition labels for the simulated cells.

Value

subDiffEx(): A vector of fdr-adjusted p-values for all genes. Nonviable results (such as for genes with 0 counts in a simulated dataset) are coerced to 1.

subDiffExttest(): A vector of fdr-adjusted p-values for all genes. Nonviable results (such as for genes with 0 counts in a simulated dataset) are coerced to 1.

subDiffExANOVA(): A vector of fdr-adjusted p-values for all genes. Nonviable results (such as for genes with 0 counts in a simulated dataset) are coerced to 1.

Functions

• subDiffEx: Get PCA components for a SCtkE object

• subDiffExttest: Runs t-tests on all genes in a simulated dataset with 2 conditions, and adjusts for FDR.

• subDiffExANOVA: Runs ANOVA on all genes in a simulated dataset with more than 2 conditions, and adjusts for FDR.

Examples

data("mouseBrainSubsetSCE")
res <- generateSimulatedData(
         totalReads = 1000, cells=10,
         originalData = assay(mouseBrainSubsetSCE, "counts"),
         realLabels = colData(mouseBrainSubsetSCE)[, "level1class"])
tempSigDiff <- subDiffEx(res)

data("mouseBrainSubsetSCE")
#sort first 100 expressed genes
ord <- rownames(mouseBrainSubsetSCE)[
  order(rowSums(assay(mouseBrainSubsetSCE, "counts")),
        decreasing = TRUE)][1:100]
#subset to those first 100 genes
subset <- mouseBrainSubsetSCE[ord, ]
res <- generateSimulatedData(totalReads = 1000, cells=10,
                             originalData = assay(subset, "counts"),
                             realLabels = colData(subset)[, "level1class"])
realLabels <- res[1, ]
output <- res[-1, ]
fdr <- subDiffExttest(output, realLabels)

data("mouseBrainSubsetSCE")
#sort first 100 expressed genes
ord <- rownames(mouseBrainSubsetSCE)[
  order(rowSums(assay(mouseBrainSubsetSCE, "counts")),
        decreasing = TRUE)][1:100]
# subset to those first 100 genes
subset <- mouseBrainSubsetSCE[ord, ]
res <- generateSimulatedData(totalReads = 1000, cells=10,
                             originalData = assay(subset, "counts"),
                             realLabels = colData(subset)[, "level2class"])
realLabels <- res[1, ]
output <- res[-1, ]
fdr <- subDiffExANOVA(output, realLabels)

mmkhan19/singleCellTK documentation built on March 22, 2022, 7:43 a.m.