inverseLogicleTransform: Computes the inverse of the transform defined by the...

In flowCore: flowCore: Basic structures for flow cytometry data

Description

inverseLogicleTransform can be use to compute the inverse of the Logicle transformation. The parameters w, t, m, a for calculating the inverse are obtained from the 'trans' input passed to the 'inverseLogicleTransform' function. (The inverseLogicleTransform method makes use of the C++ implementation of the inverse logicle transform contributed by Wayne Moore et al.)

Usage

inverseLogicleTransform(trans,transformationId,...)

Arguments

trans	An object of class 'transform' created using the 'logicleTransform' function or class 'transformList' created by 'estimateLogicle'. The parameters w, t, m, a for calculating the inverse are obtained from the 'trans' input passed to the 'inverseLogicleTransform' function.
transformationId	A name to assigned to the inverse transformation. Used by the transform routines.
...	not used.

Author(s)

Wayne Moore, N. Gopalakrishnan

References

Parks D.R., Roederer M., Moore W.A.(2006) A new "logicle" display method avoids deceptive effects of logarithmic scaling for low signals and compensated data. CytometryA, 96(6):541-51.

See Also

logicleTransform

Other Transform functions: arcsinhTransform(), biexponentialTransform(), linearTransform(), lnTransform(), logTransform(), logicleTransform(), quadraticTransform(), scaleTransform(), splitScaleTransform(), truncateTransform()

Examples

data(GvHD)
samp <- GvHD[[1]] 

#########inverse the transform object###############
logicle  <- logicleTransform(t = 10000, w = 0.5, m = 4.5 , a =0 ,"logicle")
## transform FL1-H parameter using logicle transformation
after <- transform(samp, transformList('FL1-H', logicle))

## Inverse transform the logicle transformed data to retrieve the original data
invLogicle <- inverseLogicleTransform(trans = logicle)
before <- transform (after, transformList('FL1-H', invLogicle))

#########inverse the transformList object###############
translist <- estimateLogicle(samp, c("FL1-H", "FL2-H"))
after <- transform(samp, translist)
## Inverse 
invLogicle <- inverseLogicleTransform(translist)
before <- transform (after, invLogicle)

flowCore documentation built on Nov. 8, 2020, 5:19 p.m.