lowmoi_example_data: Example low-MOI data

In Katsevich-Lab/sceptre: Analysis of Single-Cell CRISPR Screen Data

Example low-MOI data

Description

The example low-MOI data are a small simulated dataset modeled on that in the paper "Characterizing the molecular regulation of inhibitory immune checkpoints with multimodal single-cell screens" by Papalexi et al., 2021. This dataset includes gene-targeting CRISPRko perturbations as well as non- targeting perturbations.

Usage

data(lowmoi_example_data)

Format

An object of class list of length 4.

Details

The example data are stored in a list containing four components:

• response_matrix: the gene-by-cell expression matrix

• grna_matrix: the gRNA-by-cell expression matrix

• grna_target_data_frame: a data frame containing the columns grna_id (ID of an individual gRNA) and grna_target (genomic target of the gRNA)

• extra_covariates: a data frame with a single column, batch, specifying the batch in which each cell was sequenced (batch_1 or batch_2)

Katsevich-Lab/sceptre documentation built on Jan. 20, 2025, 9:03 a.m.