highmoi_example_data: Example high-MOI data

In Katsevich-Lab/sceptre: Analysis of Single-Cell CRISPR Screen Data

Example high-MOI data

Description

The example high-MOI CRISPRi data are a small simulated dataset modeled on that of "A genome-wide framework for mapping gene regulation via cellular genetic screens" by Gasperini et al., 2019. These data contain perturbations both of gene transcription start sites (TSSs) and enhancers, as well as non- targeting perturbations.

Usage

data(highmoi_example_data)

Format

An object of class list of length 4.

Details

The example data are stored in a list containing the following components:

• response_matrix: the gene-by-cell expression matrix

• grna_matrix: the gRNA-by-cell expression matrix

• extra_covariates: a data frame containing a single column, batch, specifying the batch in which each cell was sequenced (batch_1 or batch_2)

• gene_names: the human-readable name of each gene

Katsevich-Lab/sceptre documentation built on Jan. 20, 2025, 9:03 a.m.