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#' @importFrom rlang quo_is_symbol
#' @importFrom grDevices colorRampPalette
#' @importFrom RColorBrewer brewer.pal
#' @importFrom viridis viridis
#' @import dplyr
#' @import tidyr
#' @importFrom scales rescale
#' @importFrom rlang :=
#' @importFrom graphics axis
#' @importFrom graphics legend
#' @importFrom graphics par
#' @importFrom utils head
#' @importFrom stringr str_pad
#' @importFrom scales alpha
pretty_plot = function(.data,
.dim1,
.dim2,
.color=NULL,
.shape=NULL,
.size=NULL,
opacity = 1){
# Comply with CRAN NOTES
. = NULL
# Get column names
.dim1 = enquo(.dim1)
.dim2 = enquo(.dim2)
.color = enquo(.color)
.shape = enquo(.shape)
my_size_range = c(1,3)
.data_formatted =
.data %>%
# Define COLOR
when(
# If continuous
quo_is_symbol(.color) &&
(.) %>%
select(!!.color) %>%
sapply(class) %in% c("numeric", "integer", "double") ~{
order_ = findInterval( pull(.,!!.color), sort(pull(.,!!.color)))
(.) %>% mutate(.color = grDevices::colorRampPalette( viridis(n = 5) )(n())[order_] )
},
# If discrete
quo_is_symbol(.color) ~ {
how_many_colors = .data %>% distinct(!!.color) %>% nrow
(.) %>%
mutate(.color =
grDevices::colorRampPalette(RColorBrewer::brewer.pal(min(9, how_many_colors), "Set1"))(how_many_colors)[factor(!!.color)]
)
},
# If not defined
~ (.) %>% mutate(.color = "grey25")
) %>%
# Define SIZE
when(
# If not defined
is.null(.size) ~ (.) %>% mutate(.size = 2 ),
# If it is a number and not a column name
.size %>% is("numeric") ~ (.) %>% mutate(.size := !!.size ),
# If continuous
quo_is_symbol(enquo(.size)) &&
(.) %>%
select(!!enquo(.size)) %>%
sapply(class) %in% c("numeric", "integer", "double") ~ (.) %>% mutate(.size := !!enquo(.size) %>% rescale( to = my_size_range) ),
# If discrete
quo_is_symbol(enquo(.size)) ~ {
warning("tidygate says: .size has to be a continuous variable. .size has been ignored")
(.) %>% mutate(.size = 2 )
},
~ stop("tidygate says: the parameter .size must be NULL, numeric or a symbolic column name")
) %>%
# Define SHAPE
when(
# If continuous
quo_is_symbol(.shape) &
(.) %>%
select(!!.shape) %>%
sapply(class) %in% c("numeric", "integer", "double") ~ {
warning("tidygate says: .shape has to be a discrete variable. .shape has been ignored")
(.) %>% mutate(.shape = 19 )
} ,
# If discrete
quo_is_symbol(.shape) ~ (.) %>% mutate(.shape := c(19, 17, 15, 18, 3, 4, 8, 10, 5)[factor(!!.shape)] ),
# If not defined
~ (.) %>% mutate(.shape = 19 )
)
# Plot
.data_formatted %>%
{
plot(
(.) %>% pull(!!.dim1),
(.) %>% pull(!!.dim2),
xlim=range((.) %>% pull(!!.dim1)),
ylim=range((.) %>% pull(!!.dim2)),
bty='l',
pch=(.) %>% pull(.shape),
cex = (.) %>% pull(.size),
col=(.) %>% pull(.color) %>% alpha(opacity),
xlab = quo_names(.dim1) %>% paste(collapse= " "),
ylab = quo_names(.dim2) %>% paste(collapse= " "),
xaxt='n',
yaxt='n'
)
}
axis(1, tck=1, col.ticks="light gray")
axis(1, tck=-0.015, col.ticks="black", labels = FALSE)
axis(2, tck=1, col.ticks="light gray", lwd.ticks="1", las=1)
axis(2, tck=-0.015, col.ticks="black", las=1, labels = FALSE)
# Max length of the legends titles
color_title = quo_name(.color)
shape_title = quo_name(.shape)
size_title = quo_name(.size)
max_length_titles = max(nchar(color_title), nchar(shape_title), nchar(size_title))
color_title = stringr::str_pad(color_title, width = max_length_titles, side = "both")
shape_title = stringr::str_pad(shape_title, width = max_length_titles, side = "both")
size_title = stringr::str_pad(size_title, width = max_length_titles, side = "both")
# Add legend to top right, outside plot region
inset_y = 0
if( quo_is_symbol(.color)){
legend(
"topleft",
inset=c(1.05,inset_y),
legend=distinct(.data_formatted, !!.color) %>% pull(!!.color),
pch=19,
col = distinct(.data_formatted, !!.color, .color) %>% pull(.color),
title=color_title,
box.col="white",
xjust = 0
)
inset_y = inset_y + distinct(.data_formatted, !!.color, .color) %>% nrow %>% magrittr::multiply_by(.1)
}
if( quo_is_symbol(enquo(.size)) && (.data %>% select(!!enquo(.size)) %>% sapply(class) %in% c("numeric", "integer", "double") )){
legend(
"topleft",
inset=c(1.05,inset_y),
legend=distinct(.data_formatted, !!enquo(.size)) %>% pull(!!enquo(.size)) %>% range,
pch=19,
col = "black",
pt.cex = my_size_range,
title=size_title,
box.col="white",
xjust = 0
)
inset_y = inset_y + 0.3
}
if( quo_is_symbol(.shape)){
legend(
"topleft",
inset=c(1.05,inset_y),
legend=distinct(.data_formatted, !!.shape) %>% pull(!!.shape),
pch=distinct(.data_formatted, !!.shape, .shape) %>% pull(.shape),
col = "black",
title=shape_title,
box.col="white",
yjust = 0
)
inset_y = inset_y + distinct(.data_formatted, !!.shape, .shape) %>% nrow %>% magrittr::multiply_by(.1)
}
}
#' Get points within a user drawn gate
#'
#' @keywords internal
#'
#' @import dplyr
#' @import tidyr
#' @import tibble
#' @importFrom graphics plot
#' @importFrom purrr imap
#' @importFrom purrr map
#'
#' @param .data A tibble
#' @param .element A column symbol. The column that is used to calculate distance (i.e., normally genes)
#' @param .dim1 A column symbol. The x dimension
#' @param .dim2 A column symbol. The y dimension
#' @param .color A column symbol. Color of points
#' @param .shape A column symbol. Shape of points
#' @param .size A column symbol. Size of points
#' @param opacity A number between 0 and 1. The opacity level of the data points
#' @param how_many_gates An integer. The number of gates to label
#' @param name A character string. The name of the new column
#' @param ... Further parameters passed to the function gatepoints::fhs
#'
#' @return A tibble with additional columns
#'
gate_interactive_old <-
function(.data,
.element,
.dim1,
.dim2,
.color = NULL,
.shape = NULL,
.size = NULL,
opacity = 1,
how_many_gates = 1,
name = "gate", ...) {
# Comply with CRAN NOTES
. = NULL
value = NULL
# Get column names
.element = enquo(.element)
.dim1 = enquo(.dim1)
.dim2 = enquo(.dim2)
.color = enquo(.color)
.shape = enquo(.shape)
# Error if elements with coordinates are not unique
.data %>% check_data_unique(!!.element, !!.dim1, !!.dim2)
# my df
my_df =
.data %>%
select(!!.element, get_specific_annotation_columns(.data, !!.element)) %>%
distinct %>%
# Check if dimensions are NA
check_dimensions(!!.dim1, !!.dim2)
my_matrix =
my_df %>%
select(!!.element, !!.dim1, !!.dim2) %>%
.as_matrix(rownames = !!.element)
# Add extra space to right of plot area; change clipping to figure
if(
quo_is_symbol(.color) |
quo_is_symbol(.shape) |
(quo_is_symbol(enquo(.size)) && (.data %>% select(!!enquo(.size)) %>% sapply(class) %in% c("numeric", "integer", "double")))
){
# Reset par on exit
opar <- par(no.readonly =TRUE)
on.exit(par(opar))
# Set the new par
par(
mar=c(5.1, 4.1, 4.1, 8.1),
xpd=TRUE,
tck = -.01 # Reduce tick length
)
}
# Plot
my_df %>% pretty_plot(
!!.dim1,
!!.dim2,
.color = !!.color,
.shape = !!.shape,
# size can be number or column
.size = .size %>% when(is.null(.size) | is(., "numeric") ~ (.), ~ !!enquo(.)),
opacity = opacity
)
# Loop over gates # Variable needed for recalling the attributes later
gate_list = map(1:how_many_gates, ~ my_matrix %>% fhs(mark = TRUE, ...))
# Return
gate_list %>%
# Format
imap( ~ .x %>% format_gatepoints(!!.element, name, .y)) %>%
purrr::reduce(full_join, by = quo_names(.element)) %>%
unite(col = !!name,
contains(name),
sep = ",",
na.rm = TRUE) %>%
# Correct column types
# Keep classes for compatibility
imap(
~ .x %>%
when(
.y %in% colnames(my_df) &&
my_df %>% select(.y) %>% pull(1) %>% is("numeric") ~ as.numeric(.),
.y %in% colnames(my_df) &&
my_df %>% select(.y) %>% pull(1) %>% is( "integer") ~ as.integer(.),
.y %in% colnames(my_df) &&
my_df %>% select(.y) %>% pull(1) %>% is( "logical") ~ as.logical(.),
.y %in% colnames(my_df) &&
my_df %>% select(.y) %>% pull(1) %>% is( "factor") ~ as.factor(.),
~ (.)
)
) %>%
do.call(bind_cols, .) %>%
# Join with the dataset
right_join(my_df %>% select(!!.element),
by = quo_names(.element)) %>%
# Replace NAs
mutate(!!name := replace_na(!!as.symbol(name), "0")) %>%
# Add internals the list of gates
add_attr(map(gate_list, ~ attr(.x, "gate")), "gate")
}
#' Get points within a user drawn gate
#'
#' @keywords internal
#'
#' @import dplyr
#' @import tidyr
#' @import tibble
#' @importFrom graphics plot
#' @importFrom purrr imap
#' @importFrom purrr map
#'
#' @param .data A tibble
#' @param .element A column symbol. The column that is used to calculate distance (i.e., normally genes)
#' @param .dim1 A column symbol. The x dimension
#' @param .dim2 A column symbol. The y dimension
#' @param gate_list A list of gates. Each element of the list is a data frame with x and y columns. Each row is a coordinate. The order matter.
#' @param name A character string. The name of the new column
#' @param ... Further parameters passed to the function gatepoints::fhs
#'
#' @return A tibble with additional columns
#'
gate_programmatic_old <-
function(.data,
.element,
.dim1,
.dim2,
gate_list,
name = "gate",
...) {
# Comply with CRAN NOTES
. = NULL
value = NULL
# Get column names
.element = enquo(.element)
.dim1 = enquo(.dim1)
.dim2 = enquo(.dim2)
# Error if elements with coordinates are not unique
.data %>% check_data_unique(!!.element, !!.dim1, !!.dim2)
# my df
my_df =
.data %>%
select(!!.element, get_specific_annotation_columns(.data, !!.element)) %>%
distinct %>%
# Check if dimensions are NA
check_dimensions(!!.dim1, !!.dim2)
my_matrix =
my_df %>%
select(!!.element, !!.dim1, !!.dim2) %>%
.as_matrix(rownames = !!.element)
# Loop over gates # Variable needed for recalling the attributes later
gate_list_result = map(
gate_list,
~ my_matrix[applyGate(my_matrix,.x),] %>%
rownames() %>%
# Avoid error for empty gates
when(!is.null(.) ~ (.) %>% add_attr(.x, "gate") )
)
# Return
gate_list_result %>%
# Format
imap( ~ .x %>% format_gatepoints(!!.element, name, .y)) %>%
purrr::reduce(full_join, by = quo_names(.element)) %>%
unite(col = !!name,
contains(name),
sep = ",",
na.rm = TRUE) %>%
# Correct column types
# Keep classes for compatibility
imap(
~ .x %>%
when(
.y %in% colnames(my_df) &&
my_df %>% select(.y) %>% pull(1) %>% is("numeric") ~ as.numeric(.),
.y %in% colnames(my_df) &&
my_df %>% select(.y) %>% pull(1) %>% is( "integer") ~ as.integer(.),
.y %in% colnames(my_df) &&
my_df %>% select(.y) %>% pull(1) %>% is( "logical") ~ as.logical(.),
.y %in% colnames(my_df) &&
my_df %>% select(.y) %>% pull(1) %>% is( "factor") ~ as.factor(.),
~ (.)
)
) %>%
do.call(bind_cols, .) %>%
# Join with the dataset
right_join(my_df %>% select(!!.element),
by = quo_names(.element)) %>%
# Replace NAs
mutate(!!name := replace_na(!!as.symbol(name), "0")) %>%
# Add internals the list of gates
add_attr(gate_list, "gate")
}
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