pathway_heatmap: Create pathway heatmap

In ggpicrust2: Make 'PICRUSt2' Output Analysis and Visualization Easier

Create pathway heatmap

Description

This function creates a heatmap of the predicted functional pathway abundance data. The function first makes the abundance data relative, then converts the abundance data to a long format and orders the samples based on the environment information. The heatmap is then created using the 'ggplot2' library.

Arguments

abundance	A matrix or data frame of pathway abundance data, where columns correspond to samples and rows correspond to pathways. Must contain at least two samples.
metadata	A data frame of metadata, where each row corresponds to a sample and each column corresponds to a metadata variable.
group	A character string specifying the column name in the metadata data frame that contains the group variable. Must contain at least two groups.
colors	A vector of colors used for the background of the facet labels in the heatmap. If NULL or not provided, a default color set is used for the facet strips.
font_size	A numeric value specifying the font size for the heatmap.
show_row_names	A logical value indicating whether to show row names in the heatmap.
show_legend	A logical value indicating whether to show the legend in the heatmap.
custom_theme	A custom theme for the heatmap.

Value

A ggplot heatmap object representing the heatmap of the predicted functional pathway abundance data.

Examples

## Not run: 
library(ggpicrust2)
library(ggh4x)
library(dplyr)
library(tidyr)
library(tibble)
library(magrittr)

# Create example functional pathway abundance data
kegg_abundance_example <- matrix(rnorm(30), nrow = 3, ncol = 10)
colnames(kegg_abundance_example) <- paste0("Sample", 1:10)
rownames(kegg_abundance_example) <- c("PathwayA", "PathwayB", "PathwayC")

# Create example metadata
metadata_example <- data.frame(
  sample_name = colnames(kegg_abundance_example),
  group = factor(rep(c("Control", "Treatment"), each = 5))
)

# Custom colors for facet strips
custom_colors <- c("skyblue", "salmon")

# Create a heatmap using custom colors for facet strips
pathway_heatmap(kegg_abundance_example, metadata_example, "group", colors = custom_colors)

# Use real dataset
data("metacyc_abundance")
data("metadata")
metacyc_daa_results_df <- pathway_daa(
  abundance = metacyc_abundance %>% column_to_rownames("pathway"),
  metadata = metadata,
  group = "Environment",
  daa_method = "LinDA"
)
annotated_metacyc_daa_results_df <- pathway_annotation(
  pathway = "MetaCyc",
  daa_results_df = metacyc_daa_results_df,
  ko_to_kegg = FALSE
)
feature_with_p_0.05 <- metacyc_daa_results_df %>% filter(p_adjust < 0.05)
pathway_heatmap(
  abundance = metacyc_abundance %>%
    right_join(
      annotated_metacyc_daa_results_df %>%
      select(all_of(c("feature","description"))),
      by = c("pathway" = "feature")
    ) %>%
    filter(pathway %in% feature_with_p_0.05$feature) %>%
    select(-"pathway") %>%
    column_to_rownames("description"),
  metadata = metadata,
  group = "Environment",
  colors = custom_colors
)

## End(Not run)

ggpicrust2 documentation built on April 13, 2025, 9:08 a.m.