A scalable R package for dimensionality reduction and batch effect removal of single-cell RNA-seq data.
Clone this repository on your machine and than install it
install.packages("path/to/NewWave", repos = NULL, type="source")
This package depends on SharedObject 1.3.15, it is a development version that can be found on Bioconductor-devel or on the github page linked below.
If you have your data stored in a SummarizedExperiment object with batch effect variable called "batch" stored in colData then:
newWave(data,X = "~batch")
In the X matrix you can teoretically store any variable related to the cell, both quantitative and qualitative. If you a batch variable, it must be a factor. A more deteiled case of use is shown in the vignette.
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