dAB-dAB: dAB

In LinkHD: LinkHD: a versatile framework to explore and integrate heterogeneous data

Description

Function to estimate differential abundance (if nCluster in LinkData function is at least 2). The function uses a non parametric kruskal-wallis test follow up by corrected p-values. The function is robust since it doesn't assume normality on data distribution. This function calculates the differential abundance (at OTU level) betweeen all the communities data It is only used when CLusters (enterotypes-like) is activated in LinkData function. The function takes into account the compositional nature of the OTUs dataset. The differential expression is an alternative way to perform variable selection

Usage

dAB(x, Data, adjust.methods = "BH", threshold = 0.05)

Arguments

x	is an object of DistStatis Class.
Data	should be the same imput list than in LinkData object. If you integrated microbial communities and other types of data, please be careful: choose only the microbial communities as input to dab object!!!!
adjust.methods	character, correction method. Choose one between: c('holm', 'hochberg', 'hommel', 'bonferroni', 'BH', 'BY', 'fdr', 'none').
threshold	fixed pre-defined threshold value, which is referred to as the level of significance.

Value

Diferentialb: a list with selected OTUs and their p-values.

Author(s)

Laura M Zingatetti

References

2. Kruskal, W. H., & Wallis, W. A. (1952). Use of ranks in one-criterion variance analysis. Journal of the American statistical Association, 47(260), 583-621.

3. Benjamini, Y., and Hochberg, Y. (1995). Controlling the false discovery rate: a practical and powerful approach to multiple testing. Journal of the Royal Statistical Society Series B 57, 289<e2><80><93>300.

4. Wright, S. P. (1992). Adjusted P-values for simultaneous inference. Biometrics 48, 1005<e2><80><93>1013. (Explains the adjusted P-value approach.)

Examples

{
data(Taraoceans)
pro.phylo <- Taraoceans$taxonomy[ ,'Phylum']
TaraOc<-list(Taraoceans$phychem,
as.data.frame(Taraoceans$pro.phylo),as.data.frame(Taraoceans$pro.NOGs))
TaraOc_1<-scale(TaraOc[[1]])
Normalization<-lapply(list(TaraOc[[2]],TaraOc[[3]]),
function(x){DataProcessing(x,Method='Compositional')})
colnames(Normalization[[1]])=pro.phylo
colnames(Normalization[[2]])=Taraoceans$GO
TaraOc<-list(TaraOc_1,Normalization[[1]],Normalization[[2]])
names(TaraOc)<-c('phychem','pro_phylo','pro_NOGs')
TaraOc<-lapply(TaraOc,as.data.frame)
Output<-LinkData(TaraOc,Scale =FALSE,Distance =
c('ScalarProduct','Euclidean','Euclidean'),nCluster=3)
dAB(Output,Data=list(TaraOc[[2]]))
}

LinkHD documentation built on Nov. 8, 2020, 5:08 p.m.