multiHyperGeoTest: Hypergeometric tests on a list of gene sets

In HTSanalyzeR: Gene set over-representation, enrichment and network analyses for high-throughput screens

Description

This function performs hypergeometric tests for over-representation of hits, on a list of gene sets. This function applies the function hyperGeoTest to an entire list of gene sets and returns a data frame.

Usage

multiHyperGeoTest(collectionOfGeneSets, universe, hits, minGeneSetSize =
15, pAdjustMethod = "BH", verbose = TRUE)

Arguments

collectionOfGeneSets	a list of gene sets, each of which is a character vector of gene identifiers
universe	a character vector of all gene identifiers (usually all genes tested in a screen)
hits	a character vector of gene identifiers for those considered as hits
minGeneSetSize	a single integer or numeric value specifying the minimum number of elements in a gene set that must map to elements of the gene universe. Gene sets with fewer genes than this number are removed from both hypergeometric analysis and GSEA.
pAdjustMethod	a single character value specifying the p-value adjustment method to be used (see 'p.adjust' for details)
verbose	a single logical value indicating to display detailed messages (when verbose=TRUE) or not (when verbose=FALSE)

Value

a data frame containing the results of the hypergeometric test (one row per gene set)

Author(s)

John C. Rose, Xin Wang

See Also

hyperGeoTest

Examples

##example 1
gl <- runif(100, min=0, max=5)
gl <- gl[order(gl, decreasing=TRUE)]
names(gl) <- as.character(sample(x=seq(from=1, to=100, by=1), size=100,
replace=FALSE))
gs1 <- sample(names(gl), size=20, replace=FALSE)
gs2 <- sample(names(gl), size=20, replace=FALSE)
gsc <- list(subset1=gs1, subset2=gs2)
hypgeo<-multiHyperGeoTest(collectionOfGeneSets=gsc, universe=names(gl), 
hits=names(gl)[which(abs(gl) > 2)], minGeneSetSize = 2, pAdjustMethod ="BH")
##example 2
## Not run: 
library(org.Dm.eg.db)
library(KEGG.db)
##load phenotype vector (see the vignette for details about the
##preprocessing of this data set)
data("KcViab_Data4Enrich")
DM_KEGG <- KeggGeneSets(species="Dm")
##Do multiple hypergeometric tests
hypgeoResults <- multiHyperGeoTest(collectionOfGeneSets=DM_KEGG,
universe=names(KcViab_Data4Enrich), hits=names(KcViab_Data4Enrich)[which(abs(
KcViab_Data4Enrich) > 2)], minGeneSetSize = 15, pAdjustMethod = "BH")

## End(Not run)

HTSanalyzeR documentation built on Oct. 31, 2019, 7:10 a.m.