ploidy | R Documentation |
Use the FSC.A/FSC.H ratio. Diploids are typically 5um x 6um ellipsoids while haploids are typically 4um x 4um spheroids. As a result, diploids are longer and you get a larger 'area/volume' FSC.A. 'Width' might also be useful on certain cytometers.
ploidy(flowframe)
flowframe |
the flowFrame you would like to identify the ploidy of |
"Diploid" or "Haploid" and the mean FSC.A/FSC.H quotient
dat <- read.flowSet(path = system.file("extdata", "ss_example", package = "flowTime"), alter.names = TRUE) ploidy(dat$A01.fcs)
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