fcs_plot: Plot flow data from index sorted cells

In rodrigarc/RepertoiR: Scifer: Single-Cell Immunoglobulin Filtering of Sanger Sequences

Plot flow data from index sorted cells

Description

Plot a traditional flow density plot with the sorted cells and the selected thresholds for the two probes used in 'fcs_processing()'.

Usage

fcs_plot(processed_fcs_list = NULL)

Arguments

processed_fcs_list

List generated using 'fcs_processing()' containing two data.frames

Value

Returns a ggplot object with a traditional flow density plot with the sorted cells and the selected thresholds for the two probes used in fcs_processing().

Examples

index_sort_data <- fcs_processing(
    folder_path = system.file("/extdata/fcs_index_sorting",
        package = "scifer"
    ),
    compensation = TRUE, plate_wells = 96,
    probe1 = "Pre.F", probe2 = "Post.F",
    posvalue_probe1 = 600, posvalue_probe2 = 400
)

fcs_plot_obj <- fcs_plot(index_sort_data)

rodrigarc/RepertoiR documentation built on Aug. 14, 2024, 6:29 a.m.