inst/README.md

In rodrigarc/RepertoiR: Scifer: Single-Cell Immunoglobulin Filtering of Sanger Sequences

Folder organization

Inside this folder there are 3 different subfolders:

• script

  • contains igblastwrap.py, a python script based on IgDiscover to run IgBLAST in a simplified manner.
  • contains a text file describing in detail how the raw data present in extdata was generated

• rmd

  • contains Rmarkdown files that are used by quality_report() function to generate quality reports for all the sequences (HC_report.Rmd) and for individual reports for each sample (HC_individualized_report.Rmd)

• extdata

  • contains folders with raw and processed data from single-cell sorted B cell receptor sanger sequencing (sorted_sangerseq), flow cytometry data from when those cells were sorted (fcs_index_sorting), and files to test igblast wrapper (test_fasta)

    • fcs_index_sorting: .fcs files from one sample sorted in different plates. eg. E18_02 means sample E18, plate 02.

    • sorted_sangerseq: contains 2 folders for matching one of the samples in fcs_index_sorting.

      • E18_C1: contains raw .ab1 files from 12 wells sequenced by sanger sequencing
      • E18_C1_R: contains raw .ab1 files from 12 wells that were re-sequenced (repeated) to try to improve sequence quality
    • test_fasta: contains 2 fasta files, one from processing the test sequences from the sorted_sangerseq folder and another from an unprocessed fasta (test_igblast.txt).
      • KIMDB_rm: contains the KIMDB rhesus macaque B cell receptor germline database for HV, HD and HJ genes. It is used to test the sequences but it is also accessible to the user if they they BCR from rhesus macaques.

rodrigarc/RepertoiR documentation built on Dec. 19, 2024, 6:14 p.m.