Description Details Note See Also
ganalyse
is an R package that aims to make analysis on genomics
data easy. It is implemented only for RNA-Seq data at the moment.
The current organisation is as follows:
Provide experiment
, sample_info
and format
to
the function rnaseq
to generate an object of class
fpkm
or raw
.
Collect counts using gather_counts
to load the
corresponding count data for the samples. It returns an object of class
fpkm_counts
or raw_counts
respectively.
Construct design matrix
and contrasts
. See
construct_design
and construct_contrasts
.
Perform different types on analyses (only Differential Gene
Expression (DGE) analysis is supported currently) on the
fpkm_counts
or raw_counts
object.
For fpkm_counts
object, use limma_dge
to perform DGE
analysis. Similarly, for raw_counts
object, use limma_dge
or
edger_dge
methods.
The term differential gene expression or DGE
is not
used in a restrictive manner and applies to genomic features in general,
i.e., genes, transcripts, exons etc.
rnaseq
gather_counts
show_counts
construct_design
construct_contrasts
limma_dge
edger_dge
as.eset
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