geneExprTest: Prepare differential expression testing results for spike-in...

In munrosa/erccdashboard: Assess Differential Gene Expression Experiments with ERCC Controls

Description

Prepare differential expression testing results for spike-in analysis

Usage

geneExprTest(exDat)

Arguments

exDat

list, contains input data and stores analysis results

Details

This function wraps the QuasiSeq differential expression testing package for datType = "count" or uses the limma package for differential expression testing if datType = "array". Alternatively, for count data only, if correctly formatted DE test results are provided, then geneExprTest will bypass DE testing (with reduced runtime).

Examples

data(SEQC.Example)

exDat <- initDat(datType="array", isNorm=FALSE, 
                 exTable=UHRR.HBRR.arrayDat,
                 filenameRoot="testRun", sample1Name="UHRR",
                 sample2Name="HBRR", erccmix="RatioPair", 
                 erccdilution = 1, spikeVol = 50, 
                 totalRNAmass = 2.5*10^(3), choseFDR=0.01)
                 
exDat <- est_r_m(exDat)
                  
exDat <- dynRangePlot(exDat)

exDat <- geneExprTest(exDat)

munrosa/erccdashboard documentation built on April 4, 2020, 9:32 p.m.