make_gRNAs: Make gRNAs from GeCKO Library
In dbrookeUAB/GeCKO: Access and edit gRNA sequences from the GeCKO Library
Description
Usage
Arguments
Examples
Description
Make gRNAs from GeCKO Library
Usage
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make_gRNAs(seqs, gene = NULL, seq1_5p = "CACCG", seq1_3p = "",
seq2_5p = "AAAC", seq2_3p = "C")
Arguments
gene
Examples
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# Make oligos from a single sequence
seq <- "TTCCTCACCTGATGATCTTG"
gene <- "GAPDH"
make_gRNAs(seq, gene)
# Make oligos for multiple gRNA sequences
human <- get_library(species = 'human')
seq <- human[gene_id=='GAPDH', seq]
gene <- human[gene_id=='GAPDH',gene_id]
make_gRNAs(seqs = seq, gene = gene)
dbrookeUAB/GeCKO documentation built on Aug. 25, 2020, 8:35 a.m.
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Description Usage Arguments Examples
Description
Make gRNAs from GeCKO Library
Usage
1 2 | make_gRNAs(seqs, gene = NULL, seq1_5p = "CACCG", seq1_3p = "",
seq2_5p = "AAAC", seq2_3p = "C")
|
Arguments
gene |
Examples
1 2 3 4 5 6 7 8 9 10 11 12 13 | # Make oligos from a single sequence
seq <- "TTCCTCACCTGATGATCTTG"
gene <- "GAPDH"
make_gRNAs(seq, gene)
# Make oligos for multiple gRNA sequences
human <- get_library(species = 'human')
seq <- human[gene_id=='GAPDH', seq]
gene <- human[gene_id=='GAPDH',gene_id]
make_gRNAs(seqs = seq, gene = gene)
|
R Package Documentation
Browse R Packages
We want your feedback!
Note that we can't provide technical support on individual packages. You should contact the package authors for that.
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