dot-plotVolcano: Preprocess data for volcano plots and create them

In Vitek-Lab/MSstats: Protein Significance Analysis in DDA, SRM and DIA for Label-free or Label-based Proteomics Experiments

Preprocess data for volcano plots and create them

Description

Preprocess data for volcano plots and create them

Usage

.plotVolcano(
  input,
  which.Comparison,
  address,
  width,
  height,
  log_base_pval,
  ylimUp,
  ylimDown,
  FCcutoff,
  sig,
  xlimUp,
  ProteinName,
  dot.size,
  text.size,
  legend.size,
  x.axis.size,
  y.axis.size,
  log_base_FC,
  isPlotly
)

Arguments

which.Comparison	list of comparisons to draw plots. List can be labels of comparisons or order numbers of comparisons from levels(data$Label), such as levels(testResultMultiComparisons$ComparisonResult$Label). Default is "all", which generates all plots for each protein.
address	the name of folder that will store the results. Default folder is the current working directory. The other assigned folder has to be existed under the current working directory. An output pdf file is automatically created with the default name of "VolcanoPlot.pdf" or "Heatmap.pdf" or "ComparisonPlot.pdf". The command address can help to specify where to store the file as well as how to modify the beginning of the file name. If address=FALSE, plot will be not saved as pdf file but showed in window.
width	width of the saved file. Default is 10.
height	height of the saved file. Default is 10.
ylimUp	for all three plots, upper limit for y-axis. FALSE (default) for volcano plot/heatmap use maximum of -log2 (adjusted p-value) or -log10 (adjusted p-value). FALSE (default) for comparison plot uses maximum of log-fold change + CI.
ylimDown	for all three plots, lower limit for y-axis. FALSE (default) for volcano plot/heatmap use minimum of -log2 (adjusted p-value) or -log10 (adjusted p-value). FALSE (default) for comparison plot uses minimum of log-fold change - CI.
FCcutoff	for volcano plot or heatmap, whether involve fold change cutoff or not. FALSE (default) means no fold change cutoff is applied for significance analysis. FCcutoff = specific value means specific fold change cutoff is applied.
sig	FDR cutoff for the adjusted p-values in heatmap and volcano plot. level of significance for comparison plot. 100(1-sig)% confidence interval will be drawn. sig=0.05 is default.
xlimUp	for Volcano plot, the limit for x-axis. FALSE (default) for use maximum for absolute value of log-fold change or 3 as default if maximum for absolute value of log-fold change is less than 3.
ProteinName	for volcano plot only, whether display protein names or not. TRUE (default) means protein names, which are significant, are displayed next to the points. FALSE means no protein names are displayed.
dot.size	size of dots in volcano plot and comparison plot. Default is 3.
text.size	size of ProteinName label in the graph for Volcano Plot. Default is 4.
legend.size	size of legend for color at the bottom of volcano plot. Default is 7.
x.axis.size	size of axes labels, e.g. name of the comparisons in heatmap, and in comparison plot. Default is 10.
y.axis.size	size of axes labels, e.g. name of targeted proteins in heatmap. Default is 10.
isPlotly	This parameter is for MSstatsShiny application for plotly render, this cannot be used for saving PDF files as plotly do not have suppprt for PDFs currently. address and isPlotly cannot be set as TRUE at the same time.

Vitek-Lab/MSstats documentation built on Nov. 4, 2024, 3:08 p.m.