transform | R Documentation |
The transformation functions are saved in the GatingSet and can be retrieved by gh_get_transformations. Currently only flowJo-type biexponential transformation(either returned by gh_get_transformations or constructed by flowJoTrans) is supported.
## S4 method for signature 'GatingSet'
transform(`_data`, translist, ...)
_data |
|
translist |
expect a |
... |
other arguments passed to 'transform' method for 'ncdfFlowSet'.(e.g. 'ncdfFile') |
a GatingSet
or GatingSetList
object with the underling flow data transformed.
## Not run:
library(flowCore)
data(GvHD)
fs <- GvHD[1:2]
gs <- GatingSet(fs)
#construct biexponential transformation function
biexpTrans <- flowjo_biexp_trans(channelRange=4096, maxValue=262144, pos=4.5,neg=0, widthBasis=-10)
#make a transformList object
chnls <- c("FL1-H", "FL2-H")
transList <- transformerList(chnls, biexpTrans)
#add it to GatingSet
gs_trans <- transform(gs, transList)
## End(Not run)
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