flow_breaks | R Documentation |
It is mainly used as helper function to construct breaks function used by 'trans_new'.
flow_breaks(x, n = 6, equal.space = FALSE, trans.fun, inverse.fun)
x |
the raw data values |
n |
desired number of breaks (the actual number will be different depending on the data range) |
equal.space |
whether breaks at equal-spaced intervals |
trans.fun |
the transform function (only needed when equal.space is TRUE) |
inverse.fun |
the inverse function (only needed when equal.space is TRUE) |
either 10^n intervals or equal-spaced(after transformed) intervals in raw scale.
library(flowCore)
data(GvHD)
fr <- GvHD[[1]]
data.raw <- exprs(fr)[, "FL1-H"]
flow_breaks(data.raw)
trans <- logicleTransform()
inv <- inverseLogicleTransform(trans = trans)
myBrks <- flow_breaks(data.raw, equal.space = TRUE, trans = trans, inv = inv)
round(myBrks)
#to verify it is equally spaced at transformed scale
print(trans(myBrks))
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