autoGate: Automated gating of single populations in 2D

In RGLab/flowStats: Statistical methods for the analysis of flow cytometry data

Automated gating of single populations in 2D

Description

This function tries to fit a single norm2Filter based on a rough preselection of the data. This function is considered internal. Please use the API provided by lymphGate.

Usage

autoGate(x, ..., scale = 2.5)

Arguments

x	An object of class flowSet
...	Named arguments or a list of the ranges used for the initial rough preselection. This gets passed on to rectangleGate, see it's documentation for details.
scale	The scale parameter that gets passed on to norm2Filter.

Details

The flowSet is first filtered using a rectangleGate and the norm2Filter is subsequently fitted to the remaining subset.

Value

A list with items:

x	The filtered flowSet.
n2gate	The norm2Filter object.
n2gateResults	The filterResult after applying the norm2Filter on the flowSet.

Author(s)

Florian Hahne

See Also

lymphGate, norm2Filter

Examples

library(flowCore)
data(GvHD)
flowStats:::autoGate(GvHD[10:15], "FSC-H"=c(100,500), "SSC-H"=c(0, 400))

RGLab/flowStats documentation built on July 20, 2023, 1:33 a.m.