setClass("BSParams",
representation=representation(
X="BSgenome",
FUN="function",
exclude = "character",
simplify="logical",
maskList="logical",
motifList="character",
userMask="RangesList",
invertUserMask="logical"),
prototype=prototype(
exclude=character(0),
simplify=FALSE,
maskList=logical(0),
motifList=character(0),
invertUserMask=FALSE,
userMask=RangesList()
),
validity=function(object) {
msg <- TRUE
if (length(object@maskList) > 0) {
for(i in seq_len(length(object@maskList))) {
if(!(names(object@maskList)[i] %in% masknames(object@X))) {
msg <-
"the names of 'maskList' must be vector of names corresponding to the default BSgenome masks."
break
}
}
}
msg
})
bsapply <-
function(BSParams, ...)
{
if (!is(BSParams, "BSParams") ||
!isTRUE(validObject(BSParams, test=TRUE)))
stop("'X' must be a valid BSgenome object")
processSeqname <- function(seqname, ...)
{
seq <- BSParams@X[[seqname]]
if (length(BSParams@maskList) > 0) {
for(i in seq_len(length(BSParams@maskList))){
if(names(BSParams@maskList)[i] %in% masknames(BSParams@X)) {
active(masks(seq))[names(BSParams@maskList)[i]] <-
BSParams@maskList[[i]]
}
}
}
if (length(BSParams@motifList) > 0){
for(i in seq_len(length(BSParams@motifList))) {
seq <- maskMotif(seq, BSParams@motifList[i])
}
}
seqMask <- userMask[[seqname]]
if (length(seqMask) > 0) {
seqMask <- Mask(length(seq), start(seqMask), end(seqMask))
if (BSParams@invertUserMask)
seqMask <- gaps(seqMask)
if (!is.null(masks(seq)))
seqMask <- append(masks(seq), seqMask)
masks(seq) <- seqMask
}
BSParams@FUN(seq, ...)
}
if (length(seqnames(BSParams@X)) > 0)
seqnames <- seqnames(BSParams@X)
else
seqnames <- mseqnames(BSParams@X)
exclude <- unname(BSParams@exclude[nzchar(BSParams@exclude)])
pariahIndex <- unique(unlist(lapply(exclude, grep, seqnames)))
if (length(pariahIndex) > 0)
seqnames <- seqnames[- pariahIndex]
userMask <- reduce(BSParams@userMask)
if (BSParams@simplify) {
sapply(seqnames, processSeqname, ...)
} else {
GenomeData(lapply(structure(seqnames, names = seqnames), processSeqname, ...),
providerVersion = providerVersion(BSParams@X),
organism = organism(BSParams@X),
provider = provider(BSParams@X))
}
}
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