library(AnnotationHub)
library(BSgenome.Scerevisiae.UCSC.sacCer3)
context("makeEpiTxDbFromtRNAdb")
test_that("makeEpiTxDbFromtRNAdb:",{
httptest::skip_if_disconnected(url = "http://trna.bioinf.uni-leipzig.de/")
bs <- BSgenome.Scerevisiae.UCSC.sacCer3
# get tx
ah <- AnnotationHub()
edb <- query(ah, c("EnsDb","Saccharomyces cerevisiae", "99"))[[1]]
seqlevelsStyle(edb) <- "UCSC"
tx <- exonsBy(edb,"tx")
tx_id <- IRanges::CharacterList(Map(rep,names(tx),lengths(tx)))
mcols(tx, level="within")[,"tx_id"] <- tx_id
genome(tx) <- "sacCer3"
tx <- tx[lengths(tx) != 0L]
#
seq <- getSeq(bs,tx)
seq <- relist(unlist(unlist(seq)),
IRanges::PartitioningByWidth(sum(nchar(seq))))
seq_rna <- as(seq,"RNAStringSet")
expect_message(etdb <- makeEpiTxDbFromtRNAdb("Saccharomyces cerevisiae",
seq_rna))
expect_s4_class(etdb,"EpiTxDb")
expect_s3_class(metadata(etdb),"data.frame")
#
actual <- modifications(etdb)
expect_s4_class(actual,"GRanges")
expect_length(actual,2990L)
dbDisconnect(etdb$conn)
})
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