flowjo_biexp_trans: flowJo biexponential transformation.

In flowWorkspace: Infrastructure for representing and interacting with gated and ungated cytometry data sets.

Description

Used for constructing biexponential transformation object.

Usage

flowjo_biexp_trans(..., n = 6, equal.space = FALSE)

flowJo_biexp_trans(...)

Arguments

...	parameters passed to flowJoTrans
n	desired number of breaks (the actual number will be different depending on the data range)
equal.space	whether breaks at equal-spaced intervals

Value

biexponential transformation object

Examples

library(flowCore)
data(GvHD)
fr <- GvHD[[1]]
data.raw <- exprs(fr)[, "FL1-H"]
trans.obj <- flowjo_biexp_trans(equal.space = TRUE)
brks.func <- trans.obj[["breaks"]]
brks <- brks.func(data.raw)
brks # biexp space displayed at raw data scale

#transform it to verify it is equal-spaced at transformed scale
trans.func <- trans.obj[["transform"]]

print(trans.func(brks))

Example output

As part of improvements to flowWorkspace, some behavior of
GatingSet objects has changed. For details, please read the section
titled "The cytoframe and cytoset classes" in the package vignette:

  vignette("flowWorkspace-Introduction", "flowWorkspace")
[1] 8.693758e-01 1.159248e+02 3.450350e+02 1.006918e+03 3.120342e+03
[6] 1.000442e+04
[1]  459.0  927.6 1396.2 1864.8 2333.4 2802.0

flowWorkspace documentation built on Nov. 8, 2020, 8:08 p.m.