reliabilityCheckSwath: A function to check the coverage, fdr distrubtions,...

Usage Arguments Value Examples

View source: R/reliabilityCheckSwath.R

Usage

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reliabilityCheckSwath(seed.swathfile, ext.swathfile, max.fdrpass = 3, max.peptide = 2)

Arguments

seed.swathfile

A string representing the Swath results obtained using the seed library. The Swath result file should be a PeakView extracted Excel (.xlsx) file with six tabs: "Area - ions", "Area - peptides", "Area - proteins", "Score", "FDR" and "Observed RT". The SWATH result checking functions require that worksheet “Area - peptides" and “FDR" must exist.

ext.swathfile

A string representing the Swath results obtained using the extended library. The Swath result file should be a PeakView extracted Excel (.xlsx) file with six tabs: “Area - ions", “Area - peptides", “Area - proteins", “Score", “FDR" and “Observed RT". The SWATH result checking functions require that worksheet “Area - peptides" and “FDR" must exist.

max.fdrpass

A numeric value representing the maximum number of samples that pass the fdr threshold (0.01)

max.peptide

A numeric value representing the maximum number of peptides in a protein as a filter

Value

fdr.bins

a matrix of the FDR percentage in each of the 8 bins

dat.comb

a matrix of the various numbers as the SWATH filtering threshold changes. These numbers include protein, peptide, median correlation, cv and bland altman mesuarement.

Examples

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files <- paste(system.file("files",package="SwathXtend"), 
		c("Swath_result_Lib2.xlsx", "Swath_result_Lib2_3.xlsx"), sep="/")
res = reliabilityCheckSwath(files[1], files[2])

SwathXtend documentation built on Nov. 8, 2020, 6:42 p.m.