readNimblegen: Function to read in Nimblegen Intensity Text Files

Description Usage Arguments Value Author(s) See Also Examples

View source: R/readNimblegen.R

Description

Function to read in Nimblegen Intensity Text Files into an RGList. Calls some other functions for actual reading of data. This function is to be used with two-color NimbleGen array data. Use the function read.xysfiles of the oligo package for single-color data.

Usage

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readNimblegen(hybesFile, spotTypesFile, path = getwd(),
 headerPattern="# software=NimbleScan",verbose = TRUE, ...)

Arguments

hybesFile

Name of the file describing the arrays. In limma this file would be called targets file.

spotTypesFile

spot types also used by limma

path

Path to directoy that hold the files hybesFile, spotTypesFile and also the intensity files. Set this to NULL if you prefer the arguments hybesFile, spotTypesFile and the file-name entries of the hybes file to be treated as absolute or relative file paths themselves.

headerPattern

string; pattern used to identify explantory header lines in the supplied pair-format files

verbose

logical; progress output to STDOUT?

...

further arguments passed on the readNgIntensitiesTxt

Value

Returns raw intensity values in form of an RGList.

Author(s)

Joern Toedling

See Also

rglist, readTargets

Examples

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  exDir <- system.file("exData",package="Ringo")
  exRG <- readNimblegen("example_targets.txt","spottypes.txt",path=exDir)
  print(exRG)

Ringo documentation built on Nov. 8, 2020, 5:34 p.m.