R/pool.R

In vd4mmind/methylkit: DNA methylation analysis from high-throughput bisulfite sequencing results

#' Pool replicates within groups to a single sample per group
#'
#' The function sums up coverage, numCs and numTs values within each group so 
#' one representative sample for each group will be created in a new methylBase object
#' @param obj  \code{methylBase} object with two groups or more and each group 
#' should have multiple samples
#' @param sample.ids  a character vector of new sample.ids ex:c("test","control"), 
#'                     should follow the same order as unique treatment vector,
#'                    and should be equal to the length of the unique treatment
#'                     vector
#' @return a  \code{methylBase} object
#'
#' @usage pool(obj,sample.ids)
#' @author  Altuna Akalin
#' @export
#' @examples
#' library(methylKit)
#' data(methylKit)
#' 
#' # methylBase.obj has two groups, each group has two samples,
#' # the following function will pool the samples in each group
#' # so that each group will be represented by one pooled sample
#' pooled.methylBase=pool(methylBase.obj,sample.ids=c("test","control"))
#'
#' @docType methods
#' @rdname pool-methods
setGeneric("pool", function(obj,sample.ids) standardGeneric("pool"))

#' @rdname pool-methods
#' @aliases pool,methylBase-method
setMethod("pool", "methylBase",
                    function(obj,sample.ids){
  df=getData(obj)

  treat=unique(obj@treatment)
  res=df[,1:4]
  for(i in 1:length(treat) ){

     # get indices
     setCs=obj@numCs.index[obj@treatment==treat[i]]
     setTs=setCs+1
     set.cov=setCs-1

     if(length(setCs)>1){
       Cs=rowSums(df[,setCs],na.rm=TRUE)
       Ts=rowSums(df[,setTs],na.rm=TRUE)
       covs=rowSums(df[,set.cov],na.rm=TRUE)

     }else{
       Cs  =df[,setCs]
       Ts  =df[,setTs]
       covs=df[,set.cov]
     }
     res=cbind(res,covs,Cs,Ts) # bind new data
     # change names of columns
     names(res)[(2:4)+3*i]=paste( c("coverage" ,"numCs" ,"numTs"),i,sep="") 

  }
  coverage.ind=3*(1:length(treat)) + 2
  obj1=new("methylBase",as.data.frame(res),sample.ids=sample.ids,
         assembly=obj@assembly,context=obj@context,
         treatment=treat,coverage.index=coverage.ind,
         numCs.index=coverage.ind+1,numTs.index=coverage.ind+2,
           destranded=obj@destranded,resolution=obj@resolution )
  obj1

  
                    
})