library("sevenbridges")
rbx <- Tool(
id = "rnaseqGene",
label = "rnaseqgene",
description = "A RNA-seq Differiencial Expression Flow and Report",
hints = requirements(docker(pull = "tengfei/rnaseqgene"), cpu(1), mem(2000)),
baseCommand = "performDE.R",
inputs = list(
input(
id = "bamfiles", label = "bam files",
description = "a list of bam files",
type = "File...", ## or type = ItemArray("File")
prefix = "--bamfiles",
required = TRUE,
itemSeparator = ","
),
input(
id = "design", label = "design matrix",
type = "File",
required = TRUE,
prefix = "--design"
),
input(
id = "gtffile", label = "gene feature files",
type = "File",
stageInput = "copy",
required = TRUE,
prefix = "--gtffile"
),
input(
id = "format", label = "report foramt html or pdf",
type = enum("format", c("pdf", "html")),
prefix = "--format"
)
),
outputs = list(
output(
id = "report", label = "report",
description = "A reproducible report created by Rmarkdown",
glob = Expression(
engine = "#cwl-js-engine",
script = "x = $job[['inputs']][['format']]; if(x == 'undefined' || x == null){x = 'html';}; 'rnaseqGene.' + x"
)
),
output(
id = "heatmap", label = "heatmap",
description = "A heatmap plot to show the Euclidean distance between samples",
glob = "heatmap.pdf"
),
output(
id = "count", label = "count",
description = "Reads counts matrix",
glob = "count.csv"
),
output(
id = "de", label = "Differential expression table",
description = "Differential expression table",
glob = "de.csv"
)
)
)
fl <- "inst/docker/rnaseqGene/rabix/rnaseqGene.json"
write(rbx$toJSON(pretty = TRUE), fl)
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