Minor changes:
importCellCycleMarkers
, importCellTypeMarkers
: reworked internal code to
no longer use makeGeneToSymbolFromEnsembl
, which is slated to be removed in
the next AcidGenomes package update. Instead it is recommended to use
makeGRangesFromEnsembl
to generate a GRanges
object first, and then
generate GeneToSymbol
in a secondary call.CellCycleMarkers
and CellTypeMarkers
generators.CellCycleMarkers
and CellTypeMarkers
output.Minor changes:
indropsSampleIndexCounts
from koopa R package here.leftJoin
steps to ensure we don't type switch.Major changes:
convertSampleIDsToNames
to convertSampleIdsToNames
.Minor changes:
Minor changes:
diffExp
: Remove references to BiocParallel, which are now picked up
automatically in DESeq2.Minor changes:
DFrame
directly instead of DataFrame
virtual class.Minor changes:
requireNamespaces
import from AcidBase to goalie.Major changes:
export
: Reduced the number of exported methods, matching the conventions in
updated pipette v0.10.0 package. Note that con
is now required instead
of dir
for target directory.Minor changes:
diffExpPerCluster
and findMarkers
: Made the CLI messages less busy by
calling alert
instead of h1
internally.geometricMean
and zerosVsDepth
: Reworked method to dispatch on
sparseMatrix
instead of Matrix
, due to breaking changes in Matrix 1.5
release. Note that coercion to logical Matrix should now be used calling
lMatrix
instead of lgCMatrix
. See zerosVsDepth
for details.Minor changes:
Minor changes:
Major changes:
DataFrame
were updated to inherit DFrame
instead,
due to a breaking change in Bioconductor 3.15.Minor changes:
metrics
: Removed tbl_df
return option, to remove dependency on tibble
package, in favor of simply using Bioconductor S4Vectors.percent
usage in
filterCells
reporting statistics.Significantly reworked the package, migrating some single-cell RNA-seq analysis code that was previously defined in pointilllism package, but is generally applicable for any type of single-cell RNA-seq. We are reworking the pointillism package to function primarily as an extension toolkit for Seurat and monocle3.
New functions and classes:
barcodeRanksPerSample
, cellCountsPerCluster
, cpm
, diffExp
,
diffExpPerCluster
, findMarkers
, and normalize
methods that dispatch on
SingleCellExperiment
here from pointillism.CellCycleMarkers
, CellTypeMarkers
, and KnownMarkers
classes
here from pointillism.Major changes:
export
: Reworked SingleCellExperiment
method to support new BiocIO
generic approach. This method functions similarly to SummarizedExperiment
method, but also exports reducedDims
matrices to disk as well.Minor changes:
filterCells
: Package metadata is now defined as "packageName"
and
"packageVersion"
, instead of previously using just "version"
.KnownMarkers
generic from here to AcidGenerics. Previously this
was defined in pointillism package.Minor changes:
aggregate
method designed for SingleCellExperiment
. Legacy method
support for aggregateCols
is still provided, but now works as a passthrough
to aggregate
, which is defined in AcidExperiment instead of here.assay
support for filterCells
and zerosVsDepth
SingleCellExperiment methods.Minor changes:
Minor changes:
logcounts
and normcounts
as reexports.Minor changes:
Minor changes:
sampleData
.Minor changes:
Minor changes:
SingleCellExperiment-class
to reexports.Minor changes:
SingleCellExperiment
to reexports.Minor changes:
Initial release, migrating code previously defined in basejump.
Add the following code to your website.
For more information on customizing the embed code, read Embedding Snippets.