initiationScore: Get initiation score for a GRangesList of ORFs

In Roleren/ORFik: Open Reading Frames in Genomics

Get initiation score for a GRangesList of ORFs

Description

initiationScore tries to check how much each TIS region resembles, the average of the CDS TIS regions.

Usage

initiationScore(grl, cds, tx, reads, pShifted = TRUE, weight = "score")

Arguments

grl	a GRangesList object with ORFs
cds	a GRangesList object with coding sequences
tx	a GRangesList of transcripts covering grl.
reads	ribo seq reads as GAlignments, GRanges or GRangesList object
pShifted	a logical (TRUE), are riboseq reads p-shifted?
weight	a vector (default: 1L, if 1L it is identical to countOverlaps()), if single number (!= 1), it applies for all, if more than one must be equal size of 'reads'. else it must be the string name of a defined meta column in subject "reads", that gives number of times a read was found. GRanges("chr1", 1, "+", score = 5), would mean "score" column tells that this alignment region was found 5 times.

Details

Since this features uses a distance matrix for scoring, values are distributed like this:
As result there is one value per ORF:
0.000: means that ORF had no reads
-1.000: means that ORF is identical to average of CDS
1.000: means that orf is maximum different than average of CDS

If a score column is defined, it will use it as weights, see getWeights

Value

an integer vector, 1 score per ORF, with names of grl

References

doi: 10.1186/s12915-017-0416-0

See Also

Other features: computeFeatures(), computeFeaturesCage(), countOverlapsW(), disengagementScore(), distToCds(), distToTSS(), entropy(), floss(), fpkm(), fpkm_calc(), fractionLength(), insideOutsideORF(), isInFrame(), isOverlapping(), kozakSequenceScore(), orfScore(), rankOrder(), ribosomeReleaseScore(), ribosomeStallingScore(), startRegion(), startRegionCoverage(), stopRegion(), subsetCoverage(), translationalEff()

Examples

# Good hiting ORF
ORF <- GRanges(seqnames = "1",
               ranges = IRanges(21, 40),
               strand = "+")
names(ORF) <- c("tx1")
grl <- GRangesList(tx1 = ORF)
# 1 width p-shifted reads
reads <- GRanges("1", IRanges(c(21, 23, 50, 50, 50, 53, 53, 56, 59),
                            width = 1), "+")
score(reads) <- 28 # original width
cds <- GRanges(seqnames = "1",
               ranges = IRanges(50, 80),
               strand = "+")
cds <- GRangesList(tx1 = cds)
tx <- GRanges(seqnames = "1",
               ranges = IRanges(1, 85),
               strand = "+")
tx <- GRangesList(tx1 = tx)

initiationScore(grl, cds, tx, reads, pShifted = TRUE)

Roleren/ORFik documentation built on Oct. 19, 2024, 7:37 a.m.