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An implementation of methods for designing, evaluating, and comparing primer sets for multiplex PCR. Primers are designed by solving a set cover problem such that the number of covered template sequences is maximized with the smallest possible set of primers. To guarantee that high-quality primers are generated, only primers fulfilling constraints on their physicochemical properties are selected. A Shiny app providing a user interface for the functionalities of this package is provided by the 'openPrimeRui' package.
Package details |
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Author | Matthias Döring [aut, cre], Nico Pfeifer [aut] |
Bioconductor views | Coverage MultipleComparison Software Technology |
Maintainer | Matthias Döring <matthias-doering@gmx.de> |
License | GPL-2 |
Version | 1.12.1 |
Package repository | View on Bioconductor |
Installation |
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