A problem when recording 3D fluorescent microscopy images is how to properly present these results in 2D. Maximum intensity projections are a popular method to determine the focal plane of each pixel in the image. The problem with this approach, however, is that out-of-focus elements will still be visible, making edges and fine structures difficult to detect. This package aims to resolve this problem by using the contrast around a given pixel to determine the focal plane, allowing for a much cleaner structure detection than would be otherwise possible. For convenience, this package also contains functions to perform various other types of projections, including a maximum intensity projection.
Package details |
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Author | Jan Sauer, Bernd Fischer |
Bioconductor views | CellBasedAssays ImmunoOncology Preprocessing Software Visualization |
Maintainer | Jan Sauer <jan.sauer@dkfz-heidelberg.de> |
License | Artistic-2.0 |
Version | 1.11.0 |
Package repository | View on Bioconductor |
Installation |
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