Analysis of Ct values from high throughput quantitative real-time PCR (qPCR) assays across multiple conditions or replicates. The input data can be from spatially-defined formats such ABI TaqMan Low Density Arrays or OpenArray; LightCycler from Roche Applied Science; the CFX plates from Bio-Rad Laboratories; conventional 96- or 384-well plates; or microfluidic devices such as the Dynamic Arrays from Fluidigm Corporation. HTqPCR handles data loading, quality assessment, normalization, visualization and parametric or non-parametric testing for statistical significance in Ct values between features (e.g. genes, microRNAs).
Package details |
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Author | Heidi Dvinge, Paul Bertone |
Bioconductor views | DataImport DifferentialExpression GeneExpression MicrotitrePlateAssay MultipleComparison Preprocessing QualityControl Visualization qPCR |
Maintainer | Heidi Dvinge <hdvinge@fredhutch.org> |
License | Artistic-2.0 |
Version | 1.44.0 |
URL | http://www.ebi.ac.uk/bertone/software |
Package repository | View on Bioconductor |
Installation |
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